Thrombin induces GPIb-IX-mediated fibrin binding to ?IIb?3in a reconstituted Chinese hamster ovary cell model
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Propranolol induced chromosomal aberrations in Chinese hamster ovary cell line
Propranolol (PL), a non-selective beta-blocker, is a cardiovascular drug widely used to treat hypertension. The present study was concerned with assessing the cytogenetic effects of this drug on Chinese hamster ovary (CHO) cell line. MTT assay was then carried out to determine the cytotoxicity index (IC50) of the drug. The IC50 value of PL was 0.43±0.02 mM. To investigate the clastogenic effect...
متن کاملChinese hamster ovary cell culture
The effect of 26-hydroxycholesteroI and other intermediates in bile acid synthesis on HMG-CoA reductase activity was studied in Chinese hamster ovary (CHO) cell culture. Incubation of CHO cells for 5 hr in 0.25 PM 26-hydroxycholesterol caused a 40% inhibition of HMG-CoA reductase activity. All other intermediates tested including 3B-hydroxy-5cholenoic acid and cholest-5-ene-3B,7a,26-triol, oxid...
متن کاملP-22: Codon Optimization of Coagulation Factor IX and Cloning in to The Chinese Hamster Ovary Cells
Background Human coagulation factor IX is a 57kDa plasma serine protease made in Liver which plays a vital role in the blood coagulation cascade. FIX deficiency causes severe disorder Hemophilia B or Christmas disease. Nowadays, recombinant proteins have important roles in treatment of diseases. Although, cultivated mammalian cells because of their ability for producing properly folded protein ...
متن کاملA mitogen-activated protein kinase-dependent signaling pathway in the activation of platelet integrin alpha IIbbeta3.
We have recently shown that the platelet integrin alpha(IIb)beta(3) is activated by von Willebrand factor (vWF) binding to its platelet receptor, glycoprotein Ib-IX (GPIb-IX), via the protein kinase G (PKG) signaling pathway. Here we show that GPIb-IX-mediated activation of integrin alpha(IIb)beta(3) is inhibited by dominant negative mutants of Raf-1 and MEK1 in a reconstituted integrin activat...
متن کاملMouse Fyn induces pseudopodium formation in Chinese hamster ovary cells
Molecular mechanisms underlying the effects of Fyn on cell morphology, pseudopodium movement, and cell migration were investigated. The Fyn gene was subcloned into pEGFP-N1 to produce pEGFP-N1-Fyn. Chinese hamster ovary (CHO) cells were transfected with pEGFP-N1-Fyn. The expression of Fyn mRNA and proteins was monitored by reverse transcription-PCR and Western blotting. Additionally, transfecte...
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ژورنال
عنوان ژورنال: Journal of Thrombosis and Haemostasis
سال: 2006
ISSN: 1538-7933,1538-7836
DOI: 10.1111/j.1538-7836.2006.02141.x